ABSTRACT
<p><b>OBJECTIVE</b>To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluorescein isothiocyanate (FITC) after trauma-hemorrhage in mice.</p><p><b>METHODS</b>Inbred BALB/c mice (6-8 weeks old, male) were epicutaneously sensitized with FITC 12 hours, 1 day, 2 days, 4 days and 7 days after closed bilateral femur fractures combined with hemorrhage. And 5 days after sensitization, DTH was evaluated by ear swelling after a challenge of FITC. Draining lymph node cells were examined for the percentages of FITC-positive cells, cluster of differentiation (CD)11c-positive cells and major histocompatibility complex II (MHC II)-positive cells by means of flow cytometry. In vitro proliferative responses of syngeneic lymphocytes and in vivo passive transfer of DTH to naive recipients induced by isolated DCs from the draining lymph nodes were determined.</p><p><b>RESULTS</b>The time of DTH to FITC decreased more significantly in the mice with trauma-hemorrhage (12 hours to 4 days) than in the mice with sham injury. After sensitization, the relative percentages of FITC+ cells, FITC+/CD11c+ cells and FITC+/CD11c+/MHC II+ cells from the draining lymph nodes were all significantly reduced following injury. And the capacity of DCs from the draining lymph nodes in stimulating proliferative responses of lymphocytes and transferring DTH to naive recipients were also inhibited after injury.</p><p><b>CONCLUSIONS</b>Trauma-hemorrhage induces repressive DTH in mice, which may be attributed, at least partially, to the reduced trafficking of DCs into the draining lymph nodes and insufficient maturation during DC migration.</p>
Subject(s)
Animals , Male , Mice , Dendritic Cells , Physiology , Flow Cytometry , Fluorescein-5-isothiocyanate , Hemorrhage , Allergy and Immunology , Hypersensitivity, Delayed , Lymphocyte Activation , Mice, Inbred BALB C , Wounds and Injuries , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To evaluate the preventive effect of the reconstruction of prevertebral fascia on the complication of cervical vertebrae anterior approach.</p><p><b>METHODS</b>One hundred and seventy-six patients with cervical vertebrae anterior approach were divided into two groups by throwing coins, coins for the positive, patients entered the group A, coins for the negative, patients entered the group B. Eighty-seven cases of group B were treated with cervical vertebrae anterior decompression, bone grafting and internal fixation with plate and screws. In other 89 cases of group A, prevertebral fascia were covered on the surface of the plate and screws as experiment group. The patients were followed up after three weeks, three months and 12 months. The incidence of the dysphagia was as observation indicator and was statistically analyzed.</p><p><b>RESULTS</b>The followed-up period was 3 weeks, 3 months and 12 months after operation. The incidence of the dysphagia of group A was respectively 25.8%, 9.0%, 5.6%; the incidence of the dysphagia of group B was respectively 25.3%, 20.6%, 14.9%. The dysphagia in the experiment group was lower than that in contrast group, 3 months and 12 months after operation (3 months P = 0.030, 12 months P = 0.049 < 0.05).</p><p><b>CONCLUSION</b>The reconstruction of prevertebral fascia is an effective method to avoid the dysphagia of cervical vertebrae anterior approach.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Cervical Vertebrae , General Surgery , Deglutition Disorders , Fasciotomy , Postoperative Complications , Plastic Surgery Procedures , MethodsABSTRACT
Objective To study the inhibitory effects of dendritic cells modified by sCD41)gene on T lymphocytes phenotypes and cytokines production and the mechanism of inducing donor-specific immune tolerance in vitro.Methods T lymphocytes prepared with Nylon Fiber Column from Balb/c mice(as reaction cells)and DCs of different groups(as stimulation cells)were subjected to primary mixed lymphocyte culture(MLC).After incubation for 7 days,the responsiveness of the cells was de- tected by MTS method at the indicated time points,and supernatants were assayed for IFN-?,IL-2, IL-4,IL-10 by ELISA kits.On the day 5,the cultured cells were assessed for the expression of CD4, CDS,CD25 and CD69 by using flow cytometry(FCM).After secondary MLC for 5 days,the same indexes were assayed hy using the same methods.Results Dendritic cells modified by sCD40 could in- duce the hyporesponsiveness to alloantigen in primary and secondary MLC.In primary MLC,the ex- pression of CD4~+ and CD8~+ T cells and CD4~+ CD25~+,CD8~+ CD25~+,CD4~+ CD69~+,CD8~+ CD69~+ T cells in IX;modified by sCD40 group were less than those in control group(P
ABSTRACT
Objective To investigate migration of dendritic cells(DC)in orthotopic rat liver transplantation models.Methods Allogeneic models(Wistar→SD,experimental group)and syngenic models(Wistar→Wistar,control group)of rat liver transplantation were established.Graft livers and host celiac lymph nodes(n=4)of each group were sampled respectively at day 3,5,and 7 after the transplantation.The acute rejection was graded according to liver histopathological changes.The dy- namic state of DC number within graft and lymph nodes was detected by means of immunohistochemi- cal staining and image analysis.T-cell active proliferative response in lymph nodes was also studied. Results The histological examination revealed that mild to severe rejection occurred on the post-opera- tive days 5 and 7.At day 3 after transplantation,the number of S-100~+ DC in allograft was signifi- cantly increased and reached the peak at day 5,then decreased gradually at day 7.A significant num- ber of S-100~+ cells was detected in the allogeneic host lymph nodes from day 3 after transplantation, and displayed a continuous increasing trend for next several days.Active proliferation of T cells in the lymph nodes was triggered as early as day 3 after transplantation.Conclusion Allogeneic liver trans- plantation induces the accelerated migration of DC within the allograft and host lymphoid nodes.DC delivers a strong and sustained stimulation among T cells in the lymphoid nodes where effector cells are predominantly sensitized and rejected the graft eventually.